HW2NGBGX5_n01_zmtarget0523-6435u.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_zmtarget0523-6435u.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10657195
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC	123902	1.1626136145580521	TruSeq Adapter, Index 3 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	43112	0.404534213740107	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	31809	0.29847441094959787	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	30158	0.28298252964311904	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	15385	0.14436256444589782	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	13864	0.13009051631315743	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	14905	61.89769	44
TATGCCG	14910	61.759563	46
CTCGTAT	14625	61.45484	42
TCGTATG	15040	61.41133	43
GTATGCC	15380	59.803978	45
AGTCACG	15580	59.556305	28
ACGTAGA	15960	58.179977	32
CGTAGAG	16170	57.467953	33
GTCACGT	16380	56.604836	29
TAGAGAT	16235	56.46123	35
TCTCGTA	15905	56.245026	41
GTAGAGA	16780	55.00338	34
CAGTCAC	16925	54.906193	27
ACGTCTG	17345	54.160095	15
CACGTCT	17530	53.62821	14
CCGTCTT	17210	53.342377	50
AAAAGGG	15975	53.238575	70
GTCTGAA	17875	52.552998	17
ATGCCGT	17605	52.404476	47
GCTTGAA	17715	51.9054	59