Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-6435s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8907131 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC | 88962 | 0.9987727810447606 | TruSeq Adapter, Index 22 (97% over 37bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 27071 | 0.3039250236692376 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 14401 | 0.16167944537921358 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 14024 | 0.1574468816053115 | No Hit |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 9401 | 0.10554464731685209 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 10880 | 0.0 | 62.375244 | 44 |
TCGTATG | 11080 | 0.0 | 61.091396 | 43 |
CTCGTAT | 10815 | 0.0 | 61.034935 | 42 |
TATGCCG | 11095 | 0.0 | 60.946396 | 46 |
GTATGCC | 11400 | 0.0 | 59.346184 | 45 |
AGTCACG | 11590 | 0.0 | 58.979248 | 28 |
TAGCATC | 11890 | 0.0 | 54.957973 | 36 |
GGTAGCA | 12385 | 0.0 | 54.93806 | 34 |
CGGTAGC | 12430 | 0.0 | 54.93597 | 33 |
GTAGCAT | 12230 | 0.0 | 54.775475 | 35 |
ACGGTAG | 12495 | 0.0 | 54.62218 | 32 |
TCTCGTA | 12045 | 0.0 | 54.046432 | 41 |
CAGTCAC | 12650 | 0.0 | 53.981777 | 27 |
CACGGTA | 12845 | 0.0 | 53.135624 | 31 |
ACGTCTG | 13090 | 0.0 | 53.04785 | 15 |
AAAAGGG | 11825 | 0.0 | 52.68501 | 70 |
CACGTCT | 13255 | 0.0 | 52.44031 | 14 |
CCGTCTT | 13015 | 0.0 | 51.578384 | 50 |
GTCTGAA | 13615 | 0.0 | 51.104267 | 17 |
ATGCCGT | 13320 | 0.0 | 50.713238 | 47 |