FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-6435s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-6435s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8907131
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC889620.9987727810447606TruSeq Adapter, Index 22 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA270710.3039250236692376No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT144010.16167944537921358No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA140240.1574468816053115No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG94010.10554464731685209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC108800.062.37524444
TCGTATG110800.061.09139643
CTCGTAT108150.061.03493542
TATGCCG110950.060.94639646
GTATGCC114000.059.34618445
AGTCACG115900.058.97924828
TAGCATC118900.054.95797336
GGTAGCA123850.054.9380634
CGGTAGC124300.054.9359733
GTAGCAT122300.054.77547535
ACGGTAG124950.054.6221832
TCTCGTA120450.054.04643241
CAGTCAC126500.053.98177727
CACGGTA128450.053.13562431
ACGTCTG130900.053.0478515
AAAAGGG118250.052.6850170
CACGTCT132550.052.4403114
CCGTCTT130150.051.57838450
GTCTGAA136150.051.10426717
ATGCCGT133200.050.71323847