FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3357u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3357u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19983152
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC587770.29413277745172534TruSeq Adapter, Index 16 (98% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG306140.15319905488383412No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC242670.12143729878049268No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT201780.10097506139171639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC99350.051.0809544
TCGTATG100500.049.73016743
TATGCCG101600.049.43325446
CTCGTAT95500.049.0722642
GTATGCC106450.047.60843745
AGTCACC130400.039.1878628
TCTCGTA114600.039.15284741
CAGTCAC135450.038.03698727
ACGTCTG144500.036.86528815
CACGTCT148400.035.84919714
GTCTGAA149800.035.419917
CCGTCTT146250.034.19756750
AAAAGGG136400.034.02540270
TGAAAAA152000.033.64267362
CGTCCAT142700.033.57773235
TCACCCG153750.033.21380230
ACCCGTC158050.032.3537332
CACCCGT158250.032.24729531
CCAGTCA161950.032.20198426
GCTTGAA159500.031.84152459
Produced by FastQC (version 0.11.2)