FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3357u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3357u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7030
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC310.44096728307254623TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA160.2275960170697013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11379800853485066No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG80.11379800853485066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC200.006668301852.52
Produced by FastQC (version 0.11.2)