Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-3357s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8922267 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT | 111422 | 1.2488081784595775 | TruSeq Adapter, Index 19 (97% over 37bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 22133 | 0.24806475753303506 | No Hit |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 13421 | 0.15042141195729739 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 11830 | 0.13258962100103033 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 11049 | 0.12383624027391243 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 10956 | 0.12279390428463977 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 10774 | 0.120754063961547 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 13910 | 0.0 | 63.40804 | 46 |
TCGTATG | 13935 | 0.0 | 63.244045 | 45 |
CTCGTAT | 13265 | 0.0 | 63.21906 | 44 |
TATGCCG | 13990 | 0.0 | 62.918594 | 48 |
GTATGCC | 14285 | 0.0 | 61.743145 | 47 |
AGTCACG | 14640 | 0.0 | 60.509956 | 28 |
TCTCGTA | 14100 | 0.0 | 58.929466 | 43 |
ACGTGAT | 15050 | 0.0 | 58.907036 | 32 |
AATCTCG | 14170 | 0.0 | 58.391678 | 41 |
CACGTGA | 15165 | 0.0 | 58.369644 | 31 |
TCACGTG | 15200 | 0.0 | 58.188534 | 30 |
GTCACGT | 15405 | 0.0 | 57.414196 | 29 |
CAGTCAC | 15575 | 0.0 | 56.83279 | 27 |
CCAATCT | 14720 | 0.0 | 56.185516 | 39 |
CCGTCTT | 16045 | 0.0 | 54.859844 | 52 |
GCTTGAA | 16130 | 0.0 | 54.72509 | 61 |
ACGTCTG | 16430 | 0.0 | 54.386242 | 15 |
ATGCCGT | 16300 | 0.0 | 54.02308 | 49 |
GCCGTCT | 16395 | 0.0 | 53.731388 | 51 |
CAATCTC | 15430 | 0.0 | 53.7142 | 40 |