FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3357s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3357s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8922267
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT1114221.2488081784595775TruSeq Adapter, Index 19 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA221330.24806475753303506No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG134210.15042141195729739No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT118300.13258962100103033No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC110490.12383624027391243No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA109560.12279390428463977No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT107740.120754063961547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC139100.063.4080446
TCGTATG139350.063.24404545
CTCGTAT132650.063.2190644
TATGCCG139900.062.91859448
GTATGCC142850.061.74314547
AGTCACG146400.060.50995628
TCTCGTA141000.058.92946643
ACGTGAT150500.058.90703632
AATCTCG141700.058.39167841
CACGTGA151650.058.36964431
TCACGTG152000.058.18853430
GTCACGT154050.057.41419629
CAGTCAC155750.056.8327927
CCAATCT147200.056.18551639
CCGTCTT160450.054.85984452
GCTTGAA161300.054.7250961
ACGTCTG164300.054.38624215
ATGCCGT163000.054.0230849
GCCGTCT163950.053.73138851
CAATCTC154300.053.714240