FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3357s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3357s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4716093
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1376802.919365669845781TruSeq Adapter, Index 2 (97% over 36bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC85880.18209988649502884No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT59320.1257820827536692No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG53540.11352617516236427No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG52650.11163901984121177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC161400.066.8346144
CTCGTAT155550.066.6698242
TCGTATG163050.065.8999843
TATGCCG163850.065.76977546
GCCGTCT164750.065.1336349
ACGTCTG168650.065.01990515
GTATGCC165950.064.8538245
CACGTCT169200.064.6844414
AGTCACC168400.064.2019428
CAGTCAC169250.064.14834627
ATGCCGT168350.063.86621547
ACACGTC174100.063.08505613
CCGTCTT170600.063.0027250
GCTTGAA170600.062.9233359
GTCTGAA174750.062.70887417
TCTCGTA164950.062.31883641
CACCCAA174150.062.06402631
CCAGTCA175550.061.88611626
CAATCTC166200.061.61848438
TGCTTGA174000.061.49263458
Produced by FastQC (version 0.11.2)