FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3191u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3191u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21177365
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT673500.3180282343908225TruSeq Adapter, Index 15 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG319240.1507458553035281No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC230310.10875290670014895No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT229530.10838458892312618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC101400.051.0843446
TATGCCG103300.050.38179848
TCGTATG103950.049.29247345
CTCGTAT102500.048.8288244
GTATGCC114850.045.58947847
TCTCGTA124500.040.537343
AGTCACA138050.039.60225728
GTCACAT139250.039.00953729
AATCTCG136600.037.10041841
ACGTCTG148600.037.0026415
CAGTCAC149650.036.57929627
TCACATG154000.035.29579530
ATGTCAG157350.035.18932734
CACGTCT157200.035.04503314
CACATGT156000.034.97823331
GAATCTC149700.033.94734240
CGTCTGA163000.033.90459416
GTCTGAA165050.033.78035717
CCGTCTT151500.033.7287452
AGAATCT153550.033.4839839
Produced by FastQC (version 0.11.2)