FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3191u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3191u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12536525
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC1105380.8817275919762455TruSeq Adapter, Index 2 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA269590.21504364247668314No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT129330.10316255900259441No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA128630.1026041905551977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG154350.059.2743443
CGTATGC154900.059.26676644
TATGCCG155100.058.89674846
CTCGTAT149600.057.99796342
GTATGCC162050.056.52219445
ACTAATC152900.056.01392436
CTAATCT153000.055.8633837
TAATCTC158150.053.9993438
AACTAAT171900.052.71396335
CAACTAA174850.052.5456434
AGTCACC176350.052.29828
ACGTCTG180800.052.01725415
TCTCGTA165150.051.96464541
AATCTCG165100.051.6619839
CACGTCT182300.051.5312414
CAGTCAC182500.050.61234327
GTCTGAA187300.050.21006417
CGTCTGA188200.049.93275516
CCAACTA186600.049.4619833
AAAAGGG173000.049.34311770
Produced by FastQC (version 0.11.2)