FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3191s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3191s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10114479
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT3681273.639604175360886TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA1309191.294372156984062No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT661740.6542502090320224No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA658300.6508491440834472No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG328870.3251477411738163No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA307390.3039108588786432No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA199980.19771656058606676No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA189570.18742438438994236No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG165900.1640222892350659No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC152860.1511298802439552No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT149100.14741243716062882No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT147880.14620624552188996No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG128550.12709502881957638No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT110490.10923943783955653No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG109130.10789483076686403No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG108530.10730162176420555No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG103910.10273391244373536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA423100.066.9053336
CGTAATC405700.066.8761638
CGTATGC422800.066.8445246
CTCGTAT408650.066.7778144
TATGCCG423250.066.55018648
TCGTATG424000.066.4981845
GTATGCC428850.065.7627747
TAATCTC416700.064.8410340
GCCGTCT432400.064.825651
CCGTACG438100.064.76733
ACGTAAT433250.064.7404137
TCTCGTA422050.064.2592643
ATGCCGT438700.064.2303749
CCGTCTT437150.064.18526552
GTAATCT421400.064.1639
AGTCACC442650.064.1452328
CACCGTA442500.064.1100231
ACCGTAC444350.064.0286732
CAGTCAC444300.063.96184527
GCTTGAA441150.063.8698361
Produced by FastQC (version 0.11.2)