FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-3191s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-3191s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845553
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC573490.48413949099717TruSeq Adapter, Index 9 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA379810.32063509403064594No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA225020.18996158305146243No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT210510.17771226045757424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC77200.054.85687344
TCGTATG78550.053.69106343
TATGCCG79100.053.45047846
CTCGTAT78300.052.8791142
TATCTCG81100.050.53535539
GTATGCC85450.049.9289945
CATTTTA95500.044.96862834
TTATCTC91150.044.92542338
AGTCACC95900.044.78219628
TCTCGTA92500.044.34486441
ACGTCTG99900.043.97012315
CACGTCT103000.042.78285614
CAGTCAC103150.041.6346427
CCGTCTT103350.040.6376250
TTTTATC104000.040.2160536
ATGCCGT105500.040.14171247
AAAAGGG100550.039.43829770
GTCTGAA111950.039.3603617
GCCGTCT107350.038.9931649
ACCATTT110450.038.94509532
Produced by FastQC (version 0.11.2)