FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-183u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-183u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11965908
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT634730.5304486713419492TruSeq Adapter, Index 14 (97% over 44bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG144570.12081824463300236No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA123810.10346895530201303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC83450.057.8359346
TATGCCG84250.057.28674348
CTCGTAT83050.057.2711644
TCGTATG84400.057.1017645
CGTATCT83250.056.88204239
TTCCGTA87500.055.8394136
CCGTATC86850.054.88692538
TCCGTAT88700.054.3737237
GTATGCC89150.054.21658347
TATCTCG88150.053.6793141
GTATCTC96700.049.22285540
AGTTCCG101400.048.56538434
GTTCCGT101900.048.01715535
AGTCACA103450.047.70668828
TCTCGTA100800.046.9080543
ACAGTTC110500.044.56513632
CAGTCAC111450.044.34506627
ACGTCTG114550.043.4476315
TCACAGT113450.043.2546130
TGAAAAA114650.043.073464
Produced by FastQC (version 0.11.2)