Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-183u2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23625654 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC | 1434464 | 6.071637212667214 | TruSeq Adapter, Index 7 (97% over 37bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 197868 | 0.8375133234406971 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 78096 | 0.3305559287374648 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 48533 | 0.20542500114494183 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 33485 | 0.14173152624685012 | No Hit |
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA | 25210 | 0.10670604081478548 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTATG | 167665 | 0.0 | 65.81153 | 43 |
CTCGTAT | 162985 | 0.0 | 65.79021 | 42 |
CGTATGC | 167925 | 0.0 | 65.78828 | 44 |
TATGCCG | 167660 | 0.0 | 65.76269 | 46 |
GTATGCC | 169160 | 0.0 | 65.23335 | 45 |
TCTCGTA | 164580 | 0.0 | 64.56128 | 41 |
AAAAGGG | 160715 | 0.0 | 63.893154 | 70 |
AGTCACC | 173565 | 0.0 | 63.874203 | 28 |
CCGTCTT | 172590 | 0.0 | 63.662216 | 50 |
CAGTCAC | 174545 | 0.0 | 63.63977 | 27 |
GCCGTCT | 173175 | 0.0 | 63.416843 | 49 |
ATGCCGT | 174215 | 0.0 | 63.27224 | 47 |
GCTTGAA | 174385 | 0.0 | 63.24843 | 59 |
TGAAAAA | 176530 | 0.0 | 63.028984 | 62 |
CCAGTCA | 177210 | 0.0 | 62.731953 | 26 |
TGCTTGA | 175735 | 0.0 | 62.66695 | 58 |
ACGTCTG | 179380 | 0.0 | 62.60838 | 15 |
AGATCTC | 168860 | 0.0 | 62.47877 | 38 |
GTCTGAA | 180005 | 0.0 | 62.390957 | 17 |
TGCCGTC | 176580 | 0.0 | 62.370903 | 48 |