FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-183u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-183u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23625654
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC14344646.071637212667214TruSeq Adapter, Index 7 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1978680.8375133234406971No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA780960.3305559287374648No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT485330.20542500114494183No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA334850.14173152624685012No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA252100.10670604081478548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1676650.065.8115343
CTCGTAT1629850.065.7902142
CGTATGC1679250.065.7882844
TATGCCG1676600.065.7626946
GTATGCC1691600.065.2333545
TCTCGTA1645800.064.5612841
AAAAGGG1607150.063.89315470
AGTCACC1735650.063.87420328
CCGTCTT1725900.063.66221650
CAGTCAC1745450.063.6397727
GCCGTCT1731750.063.41684349
ATGCCGT1742150.063.2722447
GCTTGAA1743850.063.2484359
TGAAAAA1765300.063.02898462
CCAGTCA1772100.062.73195326
TGCTTGA1757350.062.6669558
ACGTCTG1793800.062.6083815
AGATCTC1688600.062.4787738
GTCTGAA1800050.062.39095717
TGCCGTC1765800.062.37090348