Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-183s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10625539 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT | 368761 | 3.470515707485521 | TruSeq Adapter, Index 21 (97% over 40bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 70614 | 0.6645686397650039 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 40792 | 0.3839052305958314 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 33904 | 0.3190802838331307 | No Hit |
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA | 19953 | 0.18778341503428675 | No Hit |
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG | 17493 | 0.1646316483333222 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 13944 | 0.1312309897879063 | No Hit |
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA | 12617 | 0.11874221157157298 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 41305 | 0.0 | 66.304794 | 44 |
CGTATGC | 43345 | 0.0 | 66.187355 | 46 |
TATGCCG | 43495 | 0.0 | 65.91051 | 48 |
CGTTTCG | 46435 | 0.0 | 65.908 | 33 |
TCGTATG | 43040 | 0.0 | 65.84352 | 45 |
TTTCGGA | 46625 | 0.0 | 65.676636 | 35 |
ACGTTTC | 46805 | 0.0 | 65.44619 | 32 |
CACGTTT | 46910 | 0.0 | 65.339165 | 31 |
TTCGGAA | 46780 | 0.0 | 65.25671 | 36 |
GTATGCC | 44285 | 0.0 | 65.06697 | 47 |
AGTCACG | 47100 | 0.0 | 65.03751 | 28 |
TCGGAAT | 46615 | 0.0 | 64.90986 | 37 |
GTCACGT | 47480 | 0.0 | 64.4663 | 29 |
GTTTCGG | 48035 | 0.0 | 63.785824 | 34 |
TCTCGTA | 43040 | 0.0 | 63.640083 | 43 |
CAGTCAC | 48150 | 0.0 | 63.561092 | 27 |
CCGTCTT | 44040 | 0.0 | 63.116024 | 52 |
TCACGTT | 48645 | 0.0 | 62.986855 | 30 |
ACGTCTG | 49195 | 0.0 | 62.9639 | 15 |
GCTTGAA | 44470 | 0.0 | 62.902496 | 61 |