FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-183s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-183s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10625539
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3687613.470515707485521TruSeq Adapter, Index 21 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA706140.6645686397650039No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT407920.3839052305958314No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA339040.3190802838331307No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA199530.18778341503428675No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG174930.1646316483333222No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139440.1312309897879063No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA126170.11874221157157298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT413050.066.30479444
CGTATGC433450.066.18735546
TATGCCG434950.065.9105148
CGTTTCG464350.065.90833
TCGTATG430400.065.8435245
TTTCGGA466250.065.67663635
ACGTTTC468050.065.4461932
CACGTTT469100.065.33916531
TTCGGAA467800.065.2567136
GTATGCC442850.065.0669747
AGTCACG471000.065.0375128
TCGGAAT466150.064.9098637
GTCACGT474800.064.466329
GTTTCGG480350.063.78582434
TCTCGTA430400.063.64008343
CAGTCAC481500.063.56109227
CCGTCTT440400.063.11602452
TCACGTT486450.062.98685530
ACGTCTG491950.062.963915
GCTTGAA444700.062.90249661