Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-183s2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9722353 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 121921 | 1.2540277029644984 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC | 47610 | 0.48969627002845917 | TruSeq Adapter, Index 12 (97% over 37bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 22268 | 0.22903920480978218 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 13034 | 0.13406219667193733 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 12655 | 0.13016396339445813 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 12295 | 0.12646115605964933 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 7100 | 0.0 | 56.688786 | 44 |
TATGCCG | 7290 | 0.0 | 55.73941 | 46 |
TCGTATG | 6915 | 0.0 | 55.573795 | 43 |
CTCGTAT | 6830 | 0.0 | 54.933086 | 42 |
GTATGCC | 7965 | 0.0 | 52.026382 | 45 |
ACGTCTG | 10375 | 0.0 | 48.34201 | 15 |
AGTCACC | 10395 | 0.0 | 48.049946 | 28 |
CAGTCAC | 10475 | 0.0 | 47.61639 | 27 |
CACGTCT | 10675 | 0.0 | 47.27829 | 14 |
TCTCGTA | 8010 | 0.0 | 46.27278 | 41 |
GTCTGAA | 11480 | 0.0 | 43.90161 | 17 |
ATGCCGT | 9075 | 0.0 | 42.731762 | 47 |
ACACGTC | 11935 | 0.0 | 42.521633 | 13 |
CCAGTCA | 11825 | 0.0 | 42.44667 | 26 |
CACACGT | 11940 | 0.0 | 42.328384 | 12 |
CGTCTGA | 11970 | 0.0 | 42.016754 | 16 |
CCGTCTT | 8680 | 0.0 | 41.974373 | 50 |
AAAAGGG | 9785 | 0.0 | 41.670788 | 70 |
TCGGAAG | 12440 | 0.0 | 40.87946 | 3 |
GCCGTCT | 8900 | 0.0 | 40.34714 | 49 |