FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-183s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-183s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9722353
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1219211.2540277029644984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC476100.48969627002845917TruSeq Adapter, Index 12 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA222680.22903920480978218No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA130340.13406219667193733No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT126550.13016396339445813No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA122950.12646115605964933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC71000.056.68878644
TATGCCG72900.055.7394146
TCGTATG69150.055.57379543
CTCGTAT68300.054.93308642
GTATGCC79650.052.02638245
ACGTCTG103750.048.3420115
AGTCACC103950.048.04994628
CAGTCAC104750.047.6163927
CACGTCT106750.047.2782914
TCTCGTA80100.046.2727841
GTCTGAA114800.043.9016117
ATGCCGT90750.042.73176247
ACACGTC119350.042.52163313
CCAGTCA118250.042.4466726
CACACGT119400.042.32838412
CGTCTGA119700.042.01675416
CCGTCTT86800.041.97437350
AAAAGGG97850.041.67078870
TCGGAAG124400.040.879463
GCCGTCT89000.040.3471449