FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-zmarr2u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-zmarr2u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10298399
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC297810.2891808717063691TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA181100.17585257669663024No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT105490.10243339765724749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG45150.052.40241243
CTCGTAT45050.051.50875542
TATGCCG45750.051.4856746
CGTATGC47100.050.23288744
GTATGCC50450.046.75878545
AGTCACG54250.043.80779328
GCCAATA55400.042.9605634
TATCTCG54550.042.53860539
CGCCAAT56250.042.3113833
CCAATAT57900.041.3474135
ATATCTC60300.039.06307638
TCTCGTA61900.037.88318641
CAATATC62150.037.67521736
AATATCT64150.036.60991737
ACGTCTG66350.036.39847615
GTCTGAA70050.034.62498517
GTCACGC70050.034.52639429
AAAAGGG62600.034.32895770
TGAAAAA69900.034.14999462
CAGTCAC70200.033.95419327
Produced by FastQC (version 0.11.2)