FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-zmarr2s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-zmarr2s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18728145
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT360850.19267791871538797TruSeq Adapter, Index 13 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG257610.1375523309970101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63650.045.80451245
CGTATGC65250.044.46701446
TATGCCG66050.043.7162448
CTCGTAT65650.042.91635544
GTATGCC76300.038.1191147
TCTCGTA85850.032.85906643
AGTCACA97150.030.91191128
CACAGTC98900.030.36485331
ACGTCTG103400.029.3474715
CAGTCAC105700.028.4777727
ACAGTCA105550.028.4506332
ACTCTAG80350.027.5295945
AATCTCG103100.027.42920141
CACGTCT110650.027.20314
GTCTGAA114950.026.48952917
AGTCAAC115250.026.45108434
CGTCTGA116500.026.19724816
TCACAGT116900.025.83904530
ATGCCGT115050.025.52324349
CCGTCTT115100.025.51208752
Produced by FastQC (version 0.11.2)