FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-6597u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-6597u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30584722
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG581860.19024531267604786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC504140.16483393244509464TruSeq Adapter, Index 5 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC489230.15995894943887343No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT374410.12241732980276886No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG329380.10769429259484523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90950.045.0630544
TCGTATG91750.044.5934743
TATGCCG92700.044.43873646
CTCGTAT86750.043.6133142
GTATGCC104750.039.15975645
GTCACAC128400.032.7111229
AGTCACA136400.030.79252828
ACTCTAG148150.029.5549745
TCTCGTA127400.029.28536241
CACAGTG144100.028.97667333
GACTCTA104800.028.7879184
AGTGATC132150.028.55096836
AAAAGGG136850.028.23523170
ACGTCTG154000.027.75007415
ACAGTGA153500.027.33906234
CTGACTC110100.026.9887772
ACACAGT156250.026.76794232
GCTGACT112500.026.5381571
CAGTGAT155650.026.10680635
TGAAAAA160900.026.03849462
Produced by FastQC (version 0.11.2)