HW2NGBGX5_n01_zmtarget0521-6597s.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_zmtarget0521-6597s.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23138770
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	58568	0.2531163065279615	TruSeq Adapter, Index 12 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	51699	0.2234301996173522	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	42329	0.1829353937136676	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	31756	0.13724152148104674	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	26523	0.11462579903771894	No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG	26213	0.11328605625968882	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	9375	49.728306	44
TATGCCG	9465	48.95931	46
TCGTATG	9730	47.805847	43
CTCGTAT	9565	46.544884	42
GTATGCC	10640	43.717373	45
GTAATCT	10995	40.07747	37
TAATCTC	11465	38.495327	38
TGTAATC	11625	38.146305	36
AATCTCG	11840	37.394413	39
TCTCGTA	11990	37.07279	41
AGTCACC	13080	35.80328	28
CTTGTAA	13440	34.94836	34
TTGTAAT	13555	34.470886	35
CAGTCAC	13930	33.719093	27
ACGTCTG	14190	33.6199	15
AAAAGGG	13480	32.740593	70
ACTCTAG	12380	32.511826	5
GACTCTA	8620	31.588568	4
CGTCTGA	15230	31.185421	16
CCGTCTT	15035	30.983826	50