HW2NGBGX5_n01_zmtarget0521-6435u.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_zmtarget0521-6435u.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9221952
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	29765	0.322762469377416	TruSeq Adapter, Index 4 (100% over 50bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	19345	0.2097712067900592	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	12009	0.13022188794736733	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	12006	0.13018935687368574	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	11874	0.12875798963169618	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	10036	0.10882728515611445	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	9623	0.10434884067928353	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	4280	53.07146	43
CTCGTAT	4140	52.837196	42
TATGCCG	4400	51.46497	46
GTATGCC	4670	48.639366	45
CGTATGC	5025	45.690956	44
ACTGACC	5220	43.78249	32
AGTCACT	5515	41.506702	28
TCTCGTA	5450	39.687134	41
CCAATCT	5450	39.494907	37
ACCAATC	5570	38.958206	36
CAATCTC	5745	37.710567	38
GACCAAT	6050	36.850765	35
ACGTCTG	6340	36.823288	15
AATCTCG	5910	36.479874	39
AAAAGGG	5820	36.142143	70
CTGACCA	6405	35.84634	33
GCTTGAA	6340	35.608536	59
CACTGAC	6445	35.571102	31
GTCACTG	6465	35.515972	29
CACGTCT	6665	35.132355	14