FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-6435s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-6435s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38245605
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1226060.32057539683317865TruSeq Adapter, Index 10 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG741350.1938392659757899No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC564150.14750714493861453No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT532080.13912186772833113No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG390890.10220520763104676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC177150.053.24517444
TATGCCG180400.052.49962646
TCGTATG182450.051.5258143
CTCGTAT185600.050.0667342
GTATGCC204300.046.44338245
TATCTCG203500.045.4391239
AGTCACT235000.041.82229228
GTCACTA239800.041.08726529
TCTCGTA228050.040.7622241
TTATCTC229900.040.55673638
TCACTAG241750.040.55304730
GCTTATC236750.040.2554136
CTTATCT235950.039.9025537
ACGTCTG251900.039.40527315
TAGCTTA259950.037.67275234
CACGTCT265150.037.5943814
CACTAGC262900.037.3436631
CAGTCAC266850.036.9879227
CCGTCTT263250.035.7372650
ATGCCGT272050.034.9160847