Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0521-6435s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 38245605 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC | 122606 | 0.32057539683317865 | TruSeq Adapter, Index 10 (100% over 50bp) |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 74135 | 0.1938392659757899 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 56415 | 0.14750714493861453 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 53208 | 0.13912186772833113 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 39089 | 0.10220520763104676 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 17715 | 0.0 | 53.245174 | 44 |
TATGCCG | 18040 | 0.0 | 52.499626 | 46 |
TCGTATG | 18245 | 0.0 | 51.52581 | 43 |
CTCGTAT | 18560 | 0.0 | 50.06673 | 42 |
GTATGCC | 20430 | 0.0 | 46.443382 | 45 |
TATCTCG | 20350 | 0.0 | 45.43912 | 39 |
AGTCACT | 23500 | 0.0 | 41.822292 | 28 |
GTCACTA | 23980 | 0.0 | 41.087265 | 29 |
TCTCGTA | 22805 | 0.0 | 40.76222 | 41 |
TTATCTC | 22990 | 0.0 | 40.556736 | 38 |
TCACTAG | 24175 | 0.0 | 40.553047 | 30 |
GCTTATC | 23675 | 0.0 | 40.25541 | 36 |
CTTATCT | 23595 | 0.0 | 39.90255 | 37 |
ACGTCTG | 25190 | 0.0 | 39.405273 | 15 |
TAGCTTA | 25995 | 0.0 | 37.672752 | 34 |
CACGTCT | 26515 | 0.0 | 37.59438 | 14 |
CACTAGC | 26290 | 0.0 | 37.34366 | 31 |
CAGTCAC | 26685 | 0.0 | 36.98792 | 27 |
CCGTCTT | 26325 | 0.0 | 35.73726 | 50 |
ATGCCGT | 27205 | 0.0 | 34.91608 | 47 |