FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-3357u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-3357u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920782
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC291480.22559006103500548TruSeq Adapter, Index 3 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG212360.16435537725193414No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC163490.12653258912657142No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT133360.10321356710452975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47300.048.6885444
CTCGTAT47300.047.65261542
TATGCCG48950.047.11921746
TCGTATG49350.046.8078643
ACTTAGG51800.044.93302532
GTATGCC52850.043.77439545
CACTTAG57850.040.77919831
TTAGGCA57800.040.26869634
TCACTTA58550.040.17225630
GTCACTT64800.036.24360329
TAGGCAT62800.036.05911335
AGTCACT64950.035.9982328
TCTCGTA64500.034.4567541
AAAAGGG64500.033.04681870
TGAAAAA72700.032.1596162
ACGTCTG77550.031.32177715
CAGTCAC75600.031.29743427
GTCTGAA80150.030.042917
GCTTGAA76900.029.8573559
ACTCTAG67000.029.724275
Produced by FastQC (version 0.11.2)