FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-3357s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-3357s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14591571
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC733740.5028519547346889TruSeq Adapter, Index 9 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG304020.20835316498819764No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC206860.14176677754574885No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT204720.14030017741064343No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG155280.10641760232671316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC98050.057.64872444
TCGTATG101650.055.7788343
CTCGTAT99250.055.2939242
TATGCCG103750.054.38012746
AGTCACG106950.053.082328
GTATGCC108000.052.36979745
GTCACGA115850.048.91369229
TCTCGTA112500.048.34596641
TCACGAT126150.045.1972530
ACGTCTG130150.044.45298415
CGTCTGA132850.043.78544216
ACGATCA131850.043.5338432
CACGTCT134400.043.12541214
CACGATC132350.043.0801131
CAGTCAC132250.042.95374327
AAAAGGG121700.042.8227870
ATGCCGT134500.042.1815547
GTCTGAA138350.041.8675617
CCGTCTT135550.041.80287650
ACACGTC140800.041.19002513