Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0521-3357s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14591571 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC | 73374 | 0.5028519547346889 | TruSeq Adapter, Index 9 (100% over 50bp) |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 30402 | 0.20835316498819764 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 20686 | 0.14176677754574885 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 20472 | 0.14030017741064343 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 15528 | 0.10641760232671316 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 9805 | 0.0 | 57.648724 | 44 |
TCGTATG | 10165 | 0.0 | 55.77883 | 43 |
CTCGTAT | 9925 | 0.0 | 55.29392 | 42 |
TATGCCG | 10375 | 0.0 | 54.380127 | 46 |
AGTCACG | 10695 | 0.0 | 53.0823 | 28 |
GTATGCC | 10800 | 0.0 | 52.369797 | 45 |
GTCACGA | 11585 | 0.0 | 48.913692 | 29 |
TCTCGTA | 11250 | 0.0 | 48.345966 | 41 |
TCACGAT | 12615 | 0.0 | 45.19725 | 30 |
ACGTCTG | 13015 | 0.0 | 44.452984 | 15 |
CGTCTGA | 13285 | 0.0 | 43.785442 | 16 |
ACGATCA | 13185 | 0.0 | 43.53384 | 32 |
CACGTCT | 13440 | 0.0 | 43.125412 | 14 |
CACGATC | 13235 | 0.0 | 43.08011 | 31 |
CAGTCAC | 13225 | 0.0 | 42.953743 | 27 |
AAAAGGG | 12170 | 0.0 | 42.82278 | 70 |
ATGCCGT | 13450 | 0.0 | 42.18155 | 47 |
GTCTGAA | 13835 | 0.0 | 41.86756 | 17 |
CCGTCTT | 13555 | 0.0 | 41.802876 | 50 |
ACACGTC | 14080 | 0.0 | 41.190025 | 13 |