FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-3191u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-3191u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21743993
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG398680.18335178823871034No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC333280.15327451586284083TruSeq Adapter, Index 2 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC295790.13603297241679577No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT287350.13215144063006273No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG224060.1030445512008765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60300.042.3708844
TCGTATG59900.042.1864843
TATGCCG60700.042.09185846
CTCGTAT61100.040.4414342
GTATGCC69500.036.56072245
GTATCTC78800.031.6243638
ACTCTAG116300.030.0340445
CTGACTC79800.028.9468982
TATCTCG86050.028.79687339
GACTCTA83500.028.4604844
TGTATCT88650.028.15021737
ACCGATG93800.027.76118932
GCTGACT85750.027.1426641
CACCGAT95950.026.7749131
ATGTATC95150.026.59496736
TCTCGTA94200.026.30542641
GATGTAT99600.025.82842435
ACGTCTG102250.025.6390215
AAAAGGG93750.025.23750370
CGATGTA106850.024.46915834
Produced by FastQC (version 0.11.2)