FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-3191s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-3191s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9126925
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC169910.18616346688506807TruSeq Adapter, Index 8 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG165190.18099195512179622No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT148080.16224522497993574No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC126990.13913777093599433No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG98860.10831687561802031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30800.044.20379344
TATGCCG31650.043.6801446
TCGTATG31700.042.72844743
CTCGTAT31800.041.8236542
GTATGCC37250.037.11346445
TCTCGTA40350.032.87446641
GTCACAC43800.032.76379429
AATCTCG42250.031.64443439
AGTCACA47850.029.99068528
ACGTCTG50150.029.87119315
CACACTT49750.029.1267331
CAGTCAC50200.028.72618727
TCACACT50150.028.68503230
CTGACTC43450.028.1122932
GCTGACT42850.028.0976941
CACGTCT53850.027.88345114
ACTCTAG56450.027.5287725
GAATCTC49150.027.3445538
CCGTCTT50300.027.13612250
GACTCTA46500.027.0208994
Produced by FastQC (version 0.11.2)