FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-183u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-183u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17654987
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG283250.16043625520653174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC271970.15404712560819217TruSeq Adapter, Index 1 (100% over 50bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT190000.1076183177025279No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC180970.10250361555066566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49100.042.34161844
TCGTATG50500.041.4446643
TATGCCG49950.041.3409346
CTCGTAT51150.039.4126542
GTATGCC57100.036.16426545
AGTCACA75300.028.4470628
GTCACAT77750.027.77574529
TCTCGTA74250.026.58529341
ACTCTAG85050.026.2554785
TCACGAT82450.025.46987335
GACTCTA62950.025.1306634
ACGTCTG88250.024.94656415
CAGTCAC87700.024.5845427
GCTGACT62450.024.3237691
ATCACGA89250.024.3136934
GTCTGAA92900.023.92340317
AAAAGGG82450.023.81426470
CTGACTC63400.023.6823962
TGAAAAA90550.023.4627462
CACGTCT94250.023.35845214
Produced by FastQC (version 0.11.2)