Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0521-183s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 26364696 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC | 150841 | 0.5721325214597581 | TruSeq Adapter, Index 7 (100% over 50bp) |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 51513 | 0.19538628474987915 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 42305 | 0.1604607919620996 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 37600 | 0.14261495751743164 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 36373 | 0.1379610066431261 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 27755 | 0.10527335494405093 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 19930 | 0.0 | 57.68938 | 44 |
TCGTATG | 20005 | 0.0 | 57.227615 | 43 |
TATGCCG | 20050 | 0.0 | 57.117172 | 46 |
CTCGTAT | 19610 | 0.0 | 56.488255 | 42 |
GTATGCC | 21855 | 0.0 | 52.60807 | 45 |
TCTCGTA | 22265 | 0.0 | 49.09207 | 41 |
AGTCACC | 24735 | 0.0 | 47.601807 | 28 |
AAAAGGG | 23060 | 0.0 | 45.957817 | 70 |
CAGTCAC | 25890 | 0.0 | 45.545795 | 27 |
ACGTCTG | 26555 | 0.0 | 45.524273 | 15 |
CACGTCT | 27690 | 0.0 | 43.67081 | 14 |
CGTCTGA | 27720 | 0.0 | 43.521957 | 16 |
CCGTCTT | 26385 | 0.0 | 43.19102 | 50 |
GTCTGAA | 28105 | 0.0 | 43.162296 | 17 |
ATGCCGT | 27005 | 0.0 | 42.549583 | 47 |
TGAAAAA | 27675 | 0.0 | 42.506493 | 62 |
GCTTGAA | 27030 | 0.0 | 42.407207 | 59 |
GCCGTCT | 27755 | 0.0 | 41.122295 | 49 |
CACACGT | 29475 | 0.0 | 41.10947 | 12 |
CCAGTCA | 28890 | 0.0 | 41.106987 | 26 |