FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0521-183s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0521-183s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26364696
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1508410.5721325214597581TruSeq Adapter, Index 7 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG515130.19538628474987915No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC423050.1604607919620996No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT376000.14261495751743164No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA363730.1379610066431261No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG277550.10527335494405093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC199300.057.6893844
TCGTATG200050.057.22761543
TATGCCG200500.057.11717246
CTCGTAT196100.056.48825542
GTATGCC218550.052.6080745
TCTCGTA222650.049.0920741
AGTCACC247350.047.60180728
AAAAGGG230600.045.95781770
CAGTCAC258900.045.54579527
ACGTCTG265550.045.52427315
CACGTCT276900.043.6708114
CGTCTGA277200.043.52195716
CCGTCTT263850.043.1910250
GTCTGAA281050.043.16229617
ATGCCGT270050.042.54958347
TGAAAAA276750.042.50649362
GCTTGAA270300.042.40720759
GCCGTCT277550.041.12229549
CACACGT294750.041.1094712
CCAGTCA288900.041.10698726