FastQCFastQC Report
Thu 12 Nov 2020
HW2MCDRXX_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2MCDRXX_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936168142
Sequences flagged as poor quality0
Sequence length91
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC27534600.29412024148969595No Hit
GTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTA15365670.16413365623800516No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA13050440.139402735625242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10676010.11403944997735248No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG10339350.11044330111374373No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC9525910.10175426371216978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA8111800.036.5438165
TCAACGC8697050.034.2408184
AACGCAG9183400.032.410836
CGCAGAG9355150.031.2978028
TAATGGG3728650.028.7888494
CACTAGC2858100.028.6054781
ATCAACG10757650.027.7551463
ACGCAGA10807650.027.4370887
GCAGAGT10872800.026.9069129
GTATCAA12783000.026.0466561
ACTAGCA3349800.024.6399612
TATCAAC13008200.023.7171232
CTACTCG1044150.023.160769
GGGAAGC4642550.022.954818
GTCCTAT2226650.022.9086721
GTCCTAG945000.021.8549391
TAGTCTG3119450.021.7985949
CTAGCAC4175400.021.1027373
AGAGTGA10254700.020.99464210-11
ACCTAGT3922250.020.8174021
Produced by FastQC (version 0.11.5)