FastQCFastQC Report
Thu 12 Nov 2020
HW2MCDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2MCDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936168142
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTGCG35462729837.880726985900786No Hit
TCGATATC24110875925.754856225389478No Hit
CTAGCGCT16403250717.52169291400647No Hit
TACTCATA13867258814.812786483392209No Hit
TGATCTCG73780280.7881092796255398Illumina PCR Primer Index 5 (100% over 8bp)
CGATCTCG36087690.38548299585268303Illumina Paired End PCR Primer 2 (100% over 8bp)
CTCGCGCT23407920.25003969853099317No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)