FastQCFastQC Report
Thu 12 Nov 2020
HW2MCDRXX_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2MCDRXX_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936168142
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAAT98595500.019.9025215
GCCAATA98618500.019.8882986
ATTCTAG82501300.019.8868855
TTCTAGC82421850.019.886856
TGTACAC101471300.019.885738
TACACCG99856350.019.88465310
TTCAATC78078900.019.8842376
ATGTACA101501400.019.8811347
CTTCAAT77980150.019.8806675
CTGTCCG80160900.019.8748157
GTACACC101722100.019.873299
AATAGCG98125500.019.869349
TGTCCGC80167350.019.8688498
TACTAGG60952300.019.8686
AGTCACT98911300.019.8659887
CCAATAG98787700.019.864887
GTCACTA98772000.019.8613668
TCTGTCC80293500.019.8613056
GATCCGT76532900.019.8582235
CAATCCA78018650.019.8578178
Produced by FastQC (version 0.11.5)