FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8_cw_rr.352000000a93b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8_cw_rr.352000000a93b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647585
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG429240.6457081782331479No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA291880.4390767474203037No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG201920.30374940673943995No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA151770.22830847593524567No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT139680.21012142003449374No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC126270.18994868061107906No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG118430.17815492393102156No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT117150.1762294126363183No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA105890.1592909304657255No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT103370.1555000801042785No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT95960.14435317487478536No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA94810.1426232233209504No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT93890.1412392620778824No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT91700.13794483259710105No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC90440.13604940741637753No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT89410.134499972546421No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG86780.1305436485580854No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA86670.1303781749311968No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA85930.12926498871394648No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC84670.12736956353322296No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA84290.12679792736760795No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT82230.12369905762769488No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT80770.12150277130717395No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC80140.1205550587168122No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA78180.11760661954679782No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT77830.1170801125521524No Hit
GCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGC77130.11602709856286156No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA74260.11170974120676908No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT72930.10970901462711646No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC72410.10892677566364327No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA72240.10867104369481548No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA71940.1082197519851194No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG70440.10596329343663903No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA70210.10561730312587203No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA67650.10176628053646551No Hit
GCTTAATACTCTTAGATCTTCAAATGCGGCAGAATGGCATGCCATCCACA66710.10035223317941779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA43050.0133.391771
GCAAAAG216600.0101.03593
AGCAGGC18400.093.121898
GCAGGGG53850.084.672929
GCAGGGT142250.084.5639959
AGCAAAA266150.082.54662
ACGGCCC1500.081.81625145
GGCAGCC4900.080.91717145
AGCGAAA71550.079.9591142
AGCAGGG212750.078.2747968
AAGCAGG361700.076.1318747
GAGCAAA300250.073.834961
CAAAAGC298900.073.257194
TTAGGGG1203.6379788E-1270.80253145
TATTAGT3500.066.84411
ATTAGTA6150.064.555031
TGACGCC2950.064.00228145
GGCTCGG451.3345234E-463.0151563
CGTGGGG755.3445765E-662.93558145
CTACTAA5300.060.1197286
Produced by FastQC (version 0.11.2)