FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_5-1_rr.352000000a9360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_5-1_rr.352000000a9360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6522090
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG337340.517226839862682No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA206830.3171222721550914No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG173390.26585036391708794No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA107420.1647018057095195No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC105710.1620799467655307No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG103720.15902877758509926No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA98130.15045790536469136No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA97930.1501512551958038No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT96570.14806603404736823No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG95360.1462108005255984No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT92530.14187170063583915No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG89380.13704196047585973No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT88530.13573869725808752No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT88150.13515606193720112No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA81910.1255885766679086No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT77760.11922558566349131No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT76540.11735501963327706No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC76530.11733968712483268No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT71060.10895280500575735No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC69560.1066529287391005No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT69100.10594763335065908No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA65700.10073458047957021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27350.0128.571881
GCAAAAG166400.095.686663
GCAGGGT113600.082.0172969
AGCAGGG168300.077.068928
GCAGGGG44750.076.099179
TGACGCC3500.070.14692145
AGCAAAA229750.069.953212
AAGCAGG287000.067.9160167
CAAAAGC238700.067.084484
AGCGAAA53200.066.2302552
GAGCAAA256500.063.5990941
GCGGAGC3700.059.399341
TCTACTA6650.057.55955
CACGAGC11000.056.64068
CGCGACC702.8731904E-453.95917145
AGCAGGC16950.051.7952428
ACGAGCA8250.049.773939
TATCGGG952.1643526E-549.699238145
CGTACCT2500.048.2007525
ACGAGCC4650.047.1994139
Produced by FastQC (version 0.11.2)