FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_100-1_rr.352000000a9347.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_100-1_rr.352000000a9347.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145560
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG305130.5929966806334005No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA183240.3561128429170003No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG157020.30515629008310075No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC96610.18775410256609582No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA93870.18242912336072264No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG93610.1819238333631325No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT90230.17535506339446047No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA88030.1710795326456207No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG86720.16853364842699337No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA86490.16808666112143286No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT83770.16280055037741276No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG83400.16208148384238064No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT81860.15908861231819277No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT79710.1549102527227357No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA73760.14334688547019178No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC70140.13631169396528267No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT69510.13508733743266038No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT66250.1287517782321069No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC61990.12047279596389897No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT61530.11957882135277793No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG60450.11747992443971113No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT60190.11697463444212099No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT59020.11470082945296528No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA58250.11320439369087135No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA57400.11155248408336507No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA57400.11155248408336507No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT56760.11030869332006622No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC55850.10854017832850069No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT54880.10665505795287589No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT54410.10574164911107829No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT52390.10181593451441631No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA52340.1017187633610336No Hit
GCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGC51460.1000085510614977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27050.0129.996871
GCAAAAG141500.0101.104513
GCAGGGG37000.087.6554959
GCAGGGT105450.082.93469
AGCAAAA175900.081.413342
AGCAGGG157000.077.1287168
AGCGAAA45800.076.466552
AGCAGGC9850.073.3303158
AAGCAGG253200.070.2567757
GAGCAAA217700.066.987591
CAAAAGC216200.066.5926364
ATTAGTA6850.065.203131
TGACGCC3700.063.62315145
GGCAGCC4650.062.774845145
TTAGGGG1700.060.928524145
GTACCGC652.0202463E-457.946007145
ACGGCCC1451.9135769E-951.95159145
TCTACTA6050.051.553315
TTAGTAG8950.049.9031032
AGGGTGT35300.049.1504976
Produced by FastQC (version 0.11.2)