FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_10-1_rr.352000000a9354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_10-1_rr.352000000a9354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741820
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG143710.5241408991108096No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA93710.3417802773340336No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG80430.2933452961901219No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT51910.18932679752864887No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG46730.17043423711257485No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA46620.17003304374466596No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC44560.1625197861274628No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA44350.16175387151600032No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT43490.15861726882143976No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA42850.15628305286269703No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG39430.14380958633316557No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG39100.14260600622943884No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA37100.1353115813583678No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT36240.13217497866380726No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT34780.1268500485079254No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT34250.12491702591709156No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT32750.11944620726378828No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC32510.11857087627925977No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA32080.11700257493197949No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT32070.11696610280762414No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG30410.11091173016463518No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT30050.10959873368784238No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT29410.10726451772909965No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC29290.10682685223683538No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT27490.10026186985287146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13950.0134.183621
GCAAAAG79700.099.5482563
GCAGGGT52500.087.9416669
AGCAGGG80950.080.2185448
GCAGGGG22800.080.129299
AGCAAAA101450.078.625312
AGCGAAA25050.074.723652
AAGCAGG134500.072.921867
GAGCAAA114700.070.9038541
AGCAGGC6400.070.8145148
CAAAAGC117550.068.213464
TGACGCC2300.057.335503145
GGTTACC500.00519406456.516422145
CTGCCGG1202.516208E-854.94652145
TCTACTA3300.051.5598375
TTAGGGG1304.728645E-850.719868145
TAGTAGA5300.049.498013
AGGGTGT20350.049.4144746
GGCAGCC3150.047.84459145
AAAGCAG206200.047.7027976
Produced by FastQC (version 0.11.2)