FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_1-5_rr.352000000a938a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_1-5_rr.352000000a938a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA179760.2723067820082413No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG101480.15372547974074505No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG93120.14106145716848817No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT90540.13715318225982515No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT81450.12338332996534966No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA71820.1087954666434796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9250.0114.9736941
GAGCAGA9850.084.216781
GTAACAA76100.077.228214
TATCGGG756.091068E-875.46114145
TCTCGGG5100.066.58336145
AGGATTG46800.063.7708975
AAACACG47950.062.980675
CGGTGCA13650.061.2893682
ACGAGCA31050.060.2011269
ACACGAG46950.060.177737
CGGAGCA10900.059.8407632
GCGGAGC13100.059.5347251
AGTAGCC2900.058.54744145
CACGAGC47700.058.4861878
GCAGGGT47950.057.2935079
AGCAGGG66300.056.7095838
TAACAAG105900.056.3640825
TAGAAAC80400.052.7328072
GGATTGA56700.052.5772136
TAGTAGA11500.052.402293
Produced by FastQC (version 0.11.2)