FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_1-1_rr.352000000a937d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_1-1_rr.352000000a937d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6094072
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG208780.34259523024998717No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA129390.21232108842822991No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG107300.1760727474174903No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA105230.17267600382798234No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA69370.11383193372182016No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC68910.1130771018130406No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG65120.10685794326026998No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA62290.10221408608234364No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA61480.10088492554731877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA18250.0126.262791
GCAAAAG125450.079.968553
GCAGGGT80600.076.335959
AGCAGGG114750.072.315278
GTAACAA49500.068.6017464
GCGGAGC7600.066.236851
TGACGCC3100.063.905933145
CAAAAGC165400.061.077924
GTACCGT350.00391736860.6917576
GAGCAGA6250.060.1243931
GTCGCCC2600.058.053738145
CACGAGC25900.056.3148048
AAGCAGG218300.056.1776477
ACGAGCA17350.053.458439
GCAGGGG40050.052.151159
CGGAGCA6950.052.0275152
GCGAACC1651.03682396E-1051.456726145
TAGTAGA9650.051.428543
ACGAGCC10600.050.763579
AGCGAAA45450.050.074762
Produced by FastQC (version 0.11.2)