FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_1-10_rr.352000000a9397.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_1-10_rr.352000000a9397.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262659
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA174070.3307643531530354No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG103390.196459622407608No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT89860.170750185410075No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT78210.14861308703452No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA64600.12275163562754114No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC57000.10831026673018336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4600.0100.1799551
AGGATTG30800.084.236015
GAGCAGA7900.083.460541
GTAACAA72450.083.2650454
TTGCGCC1051.07320375E-1071.85878145
GAAACAC42000.070.381474
GCGGAGC11800.069.694951
AAACACG42150.069.457625
ACGTGCA3250.067.621862
CGGTGCA10400.067.485522
TAGAAAC55150.064.273692
ACACGAG42850.063.9498757
AACACGA44550.061.8286446
GGATTGA42050.061.799926
TAACAAG98600.061.540965
ACGAGCA31500.058.8910989
CGGAGCA10150.058.6708032
TGACGCC3250.058.039787145
CACGAGC47650.057.0599488
GGACCCC1351.0004442E-955.89017145
Produced by FastQC (version 0.11.2)