FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_pr8-vww_1-100_rr.352000000a93a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_pr8-vww_1-100_rr.352000000a93a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852777
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA209430.3578301377277829No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG109910.18779119723850746No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT101530.17347320767560423No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT93400.1595823657726922No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC74120.126640738234175No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA71730.12255720660465964No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC66920.11433888562642999No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT66470.11357001983844592No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT65780.11239109229687035No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA65770.11237400639047071No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA64840.11078501709530365No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT64690.11052872849930896No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT63230.10803418616496067No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT63220.10801710025856101No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC60940.10412151359944176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG28750.0108.9810265
GCGTCAG600.0106.344131
GAAACAC35500.092.8527454
GAGCAGA9600.090.097111
GTAACAA84450.085.8709264
CGGTGCA8500.085.07022
TTCGGAA2300.082.0197145
GGATTGA42100.074.3407756
TATCGGG1650.074.31482145
TAGAAAC48300.072.359572
AACACGA43200.071.7922066
ACGTGCA4750.071.638062
GCACGCA1900.070.891842
CACGCAC2050.069.160993
AAACACG47850.068.887035
GAGCATC18000.068.5246664
GGACGTT703.566971E-667.37332145
GCGGAGC15500.067.236931
ACACGAG46000.067.112667
AACGGCC1550.066.93865145
Produced by FastQC (version 0.11.2)