FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_ny238_rr.352000000a93cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_ny238_rr.352000000a93cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738658
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG62460.22806790771246355No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA61160.2233210572477469No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG57110.2085327923384373No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT50650.1849445969522299No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT47480.17336958466519004No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT46770.17077707402676784No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG43610.15923857597407198No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT42590.1555141240709866No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA41120.15014653162242236No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC38730.1414196296142125No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC31030.11330366916935228No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG29500.10771699131472423No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT29240.10676762122178089No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG29050.10607385076924537No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG27880.10180168535100038No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC27550.10059671561764923No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT27420.10012203057117756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCC2900.051.890427145
TACTGAT22850.046.488197
CGTAGGG1054.002788E-544.786377145
TCAACGC857.6336577E-444.25948145
AATATTT3700.043.212803145
TACCGCC2404.1873136E-935.269276145
TATACTG37700.035.1646585
GTATACC6200.033.122879
AGAGCGT5800.032.9659358
ATACATC14350.032.6084373
CATATAC37300.032.122983
TAATGTA6200.032.0163965
TCGGACA1602.2376153E-631.0158844
TTCATAT38100.030.5202851
GAGCGTT6300.030.3490379
ACTGATG37050.030.1994138
ATACGCC1250.0050680330.096447145
GTATTAA6650.029.8542441
CTACCCC4101.8189894E-1229.821177145
GTCGGCC1903.7397287E-529.700441145
Produced by FastQC (version 0.11.2)