FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_extraction_neg_ctrl_rr.352000000a933a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_extraction_neg_ctrl_rr.352000000a933a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451260
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC12920.2863094446660462No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT9750.21606169392368035No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT8270.18326463679475247No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC7290.16154766653370564No Hit
ATCTGCAACAGATCAAAGAAACAAAAGTGAAAACATGGAAGCCAGGGATG6340.14049550148473164No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA5890.1305234233036387No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC5800.12852900766742012No Hit
GTATTAACTAGTTTACAGGAAATGAAGCACTTCGTACTGTGCATTTGCAT5240.11611931037539334No Hit
ATAATATTCCTACCTGTGACTAATTTGGTGTCTGTAGGGTGGAGTCTGAA5050.11190887736559854No Hit
AGTTAATACATTGGCTGTGGTGATGACTATGGGAACACTGGGGATTTTTT5020.11124407215352568No Hit
GCATAAGACAATCAATTTTGGGGGGAAAAAATCCCCAGTGTTCCCATAGT4960.10991446172937996No Hit
CTCTAGTGGAGAATTTTAATTCAGGGCTAGTGCAGAATCATTTTTTTCTG4810.10659043566901565No Hit
GTTTACGGTAATCCTGCTATTACTGTCACCTCCAAATTCCTGCATAAGAC4760.10548242698222754No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC4720.10459602003279707No Hit
GTCAAAGAACAGATATCGAACTACATAGAAAGTGAAACTGTGGGCCGGGC4690.10393121482072419No Hit
GTATGTGGATATGAACATTAACATTTTTAAGATGCAAGTACTGAAACGTA4590.10171519744714798No Hit
ACATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATC4530.10038558702300227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCC304.32695E-6125.731125145
GCTGCCC557.072231E-9102.87092145
GCGTGGC451.4187954E-678.66339
AAGAGCG1200.076.6967249
CAGCGTG502.6518828E-670.8048257
AGCGTGG502.6518828E-670.8048258
ACAGCGT554.6666046E-664.375176
CGGGTGG450.003404869762.865562145
GGATAGG755.36918E-662.865562145
GGTGGGG450.003404869762.865562145
GCACAGA500.00516764156.579006145
AGCACGC2100.054.036441
GCACGCA2000.053.1921232
AGTAACA14400.052.6996043
TAACAAG15300.049.5886085
ACGCACT2250.047.271394
GTAGTAA16950.046.8634641
GCAGGCA1850.045.993784
GTAACAA16650.045.567914
AACACGT1454.838512E-1043.9526986
Produced by FastQC (version 0.11.2)