FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_428_rr.352000000a9310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_428_rr.352000000a9310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535549
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC56440.10195917333583354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC107000.090.573782
GTAGTAA111950.086.889531
CGCACTT31800.083.1488345
GTAACAA126150.076.761914
TAACAAG148600.065.116095
CTCGCTC29250.058.4113273
TCTCGCT29150.058.126882
TCGCTCT30900.055.2892764
AGTAACA177850.054.689783
AAGAGCG21500.052.6882329
GCACGCA50450.052.556712
CGCTCTG32850.052.222115
TCTGTCG34050.051.775728
GTCTCGC35800.048.5199621
ACAAGAG204750.047.2387247
ACGAGCG4950.047.1998759
ACGCACT60500.044.6428534
AACAAGA220050.043.9864126
CTGTCGT32750.043.0203449
GACGAAA9700.042.819214145
Produced by FastQC (version 0.11.2)