FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_425_rr.352000000a9303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_425_rr.352000000a9303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372173
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT151040.2370306016487625No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG147450.2313967307541713No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC134610.21124661869663616No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT114960.1804094144964363No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA112440.1764547196066397No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA109900.17246863824946373No Hit
GTAGAAACACGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC107050.167996066647908No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC85920.13483626386163713No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC85440.13408298864453302No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT82850.13001844111890873No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG82460.12940640500501163No Hit
CCCATATTCCAGGCCCTCCCACAGCCCTCACCCGGTCAGTCACCCCATCT82230.12904546063014924No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG77710.12195211900241879No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC76150.11950397454683043No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA74680.11719706919444904No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC74230.11649087367841394No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG72390.11360331867951481No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA72030.11303836226668673No Hit
GTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATCAATTCTTTTAC69910.10971139672447687No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATC69750.10946030498544218No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT69180.10856579066513103No Hit
GTCTTGGGCAGGGCCTGAGAATGTGCGTTTCTAACCAGTTCCCAGGTGAT68860.10806360718706162No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA68820.10800083425230295No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG68500.10749865077423354No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT68470.10745157107316453No Hit
GTCCAGAAGAGGGTGAAAGGAACCTCCACTTCGGCCATTGTGGAGGCTTA66980.10511327925340382No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG65730.10315162504219517No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT64390.10104873172777952No Hit
AATTAAAACAAGGGGTGATGGGAAGAATCACAGGTTGTGGCCCCGGGGTT64320.10093887909195184No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA63830.10016991064115804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA97350.077.189484
TTCGGAA2850.076.14489145
CGCACTT17150.076.0556955
GCACGCA18000.074.048222
AGCACGC19300.069.42841
TAGTAAC118600.065.337842
TAACAAG115650.065.035095
AAACACG55800.062.6311955
TACGCGG1750.059.307888145
GAAACAC59950.058.8882184
ACACGAG53000.056.3792727
AGTAACA136950.056.375223
ACAAGAG136600.055.2054337
CGGTGCA10850.054.8881762
AACACGA55750.053.726516
ACGTGCG9300.053.3635062
AAGAGCG45650.053.356459
ACGATCC3050.052.590454145
ACGAGCC7200.052.1209839
CACGAGC56800.052.107548
Produced by FastQC (version 0.11.2)