FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_412_rr.352000000a92f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_412_rr.352000000a92f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440985
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG192530.353851370661746No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG86560.15908884145058294No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT82270.151204239673515No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA78430.14414669402690874No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT75290.13837568013879842No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG73750.13554531027010736No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG72790.13378092385845577No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA71980.13229222282362477No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG68180.12530819327750398No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT65860.12104425944934602No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC55460.10193007332312072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGG3700.0122.41976145
CGGTGCA17950.0100.317832
AGCACGC7450.090.403281
GGTGCAT21450.084.277913
CGCACTT8650.076.211365
GCGGAGC8500.075.065511
TCTCGGG5650.073.48797145
ACGCACT9300.071.64964
GCACGCA9400.070.139722
GTAACAA99600.064.5530554
GAAACAC33050.064.345574
GCGGTGC34950.063.8969651
AAACACG35650.060.0482375
AACACGA33750.057.2724726
ACACGAG34750.055.827587
GCAGAGC61450.054.8010141
TATCGGG1401.375156E-953.92299145
TAGTAAC123950.053.0202872
TAACAAG122450.052.6208575
ACGTGCA3800.052.2376142
Produced by FastQC (version 0.11.2)