FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_395_rr.352000000a92db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_395_rr.352000000a92db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4477045
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT130310.2910625200327448No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA110680.24721663507961167No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT78720.1758302630418055No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT78320.1749368165832597No Hit
GGAACAGGCTACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAA66820.14925023090006914No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC60820.13584853402188274No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT52740.1178009155592584No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51280.11453983598556637No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC48970.10938018268746462No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT48220.10770497057769131No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC47500.10609676695230895No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC46960.10489061423327216No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG46090.10294736818593514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA61100.0104.893574
CGGTGCA11500.096.1784062
AAACACG27850.095.202555
GAAACAC28250.094.109724
AGCACGC4350.083.128741
TAGAAAC35500.082.08512
GGTGCAT13500.081.402743
ACGCACT4600.080.143294
GCGCAAA2500.075.48068145
TTCGGAA3000.075.48068145
CGGAGCA13200.075.197762
TAACAAG86600.074.085445
TAGTAAC87500.073.898842
GTCTCGC1250.073.740081
AACACGA34450.073.581936
ACGTGCA3900.072.718722
GAGCAGA13900.072.434231
CGCACTT5000.072.310675
GCGGAGC16400.070.0392461
GCACGCA5300.068.225252
Produced by FastQC (version 0.11.2)