FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_379_rr.352000000a92b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_379_rr.352000000a92b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007680
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT84090.2098221414883424No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT72500.1809026668795912No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT69420.17321742254870648No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT68460.17082202171830085No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA64240.16029223890130948No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC59270.1478910491855637No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT56950.1421021638454168No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT56570.14115398435004792No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG49430.1233381906739061No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC48830.12184106515490259No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47850.11939576014053019No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT45370.113207641328649No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43740.10914045033535612No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT40860.10195424784413924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA2600.0126.733116145
CGGTGCA9150.0103.065262
GTAACAA53550.0102.6104054
GAAACAC24750.0100.549774
GGTGCAT9650.098.458633
GAGCAGA7850.098.455191
AAACACG25950.094.8036355
TAGAAAC29850.087.889942
GAGCCCC1850.081.42236145
CACGCAC1700.079.2466663
GCACGCA1750.076.9834442
AACACGA30600.073.826716
TAACAAG75150.073.68095
TAGTAAC78300.071.539692
GCGTCAG407.441734E-570.90581
AGCACGC1900.070.905791
GCCGACC2500.067.78412145
ACACGAG33500.067.646297
ACAAGAG82600.066.959147
GCGGAGC12900.065.958881
Produced by FastQC (version 0.11.2)