FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_363_rr.352000000a92a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_363_rr.352000000a92a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6554125
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATGCTGCCTACAAGAAACTCACTTCACCTACAAAGACATACATAG93100.14204794690366754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC16500.057.495749
AAACACG40550.055.5860255
GACGAAA10950.053.49441145
CGCACTT11250.051.6643145
ACACGAG37900.050.6249777
ACGCACT13400.050.253324
CACGAGC38200.050.0405278
ACGAGCT10300.048.8018159
ACGAGCG2400.047.1979989
GCCGACC3450.046.554394145
AACACGA40950.046.5085876
CAGCACG13400.042.3185844
GCACGCA16300.041.3182332
CACGCAC18400.037.3723
ATAGTAC40100.033.5452278
TAGTACC39100.033.4973039
CGTGCGC850.003062019133.3605733
GCACGCT17650.032.893036
GCGGTGC4500.031.4653329
TGACGCC13200.030.776932145
Produced by FastQC (version 0.11.2)