FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_361_rr.352000000a9298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_361_rr.352000000a9298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228559
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT392340.7503788328677174No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC157360.3009624640364582No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC98220.1878529055519886No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG92030.1760140795963094No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG80540.15403861752348974No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT77610.1484347790662781No Hit
GTGCATCAAACATTTAGTAAATATTACTCGTAGTGCTTTTCCAAAGGATC65430.12513964172537786No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC65330.1249483844401488No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT64110.1226150455603542No Hit
GTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT59890.11454398812368763No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT59780.11433360510993565No Hit
GGATCCTTTTACTAGTTTTCCTTCTTGGGTTCCAACACTGAAAGTTTTGG59470.11374070752572553No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG57450.1098773103640984No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT56100.10729533701350602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG21150.075.416935
AACACGA22900.064.007096
ACACGAG23500.062.6717837
CGGTGCA6300.059.6449552
CACGAGC25300.057.930288
TGACGCC3150.056.891617145
ACGAGCC7750.055.728819
TTATTCA111200.055.01249
GCACGCA3200.053.1740382
GAAACAC30150.053.140064
GTGTCCT116050.052.4801061
CGCACTT3550.051.9208455
TCGCGCC754.061766E-450.30416145
AGAAGCG15700.046.508715
TTTATTC135750.045.5334138
CACGCAC3950.044.8708843
ACGCACT3950.044.8687324
AGCACGC3800.044.7789961
AAGCGGT13800.044.6392367
ACCGCCC857.527904E-444.38603145
Produced by FastQC (version 0.11.2)