FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_338_rr.352000000a928b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_338_rr.352000000a928b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522606
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG62900.11389550512928136No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT59920.10849950186560475No Hit
CGATTGGAGTTATCATTGTTCCTTTCAATTTTTCTCTTTTTTCTTTTATC59310.10739495086196625No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG57650.10438912354058935No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG56900.10303106902791906No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC350.00125256480.90905145
TACGCGG1006.91216E-1175.515114145
TACGCGC505.4470293E-575.515114145
GCCGACC3800.072.037445145
AAACACG19350.068.135625
ACACGAG17350.067.333977
AACACGA17800.065.6317146
CACGAGC18950.061.6471068
ACGAGCC7800.060.814949
GAGCAGA19850.059.28611
AGCACGC2100.057.3898241
CGCACTT2200.054.773245
ACGCACT2350.054.294364
GAAACAC24100.054.119144
CGATTGG21550.053.6223261
TGACGCC5000.052.86058145
AGAAGCG13500.052.505945
CGGTGCA5500.048.978612
CAGTGCG1750.048.6054955
CGCACCC1408.337338E-847.196945145
Produced by FastQC (version 0.11.2)