FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_337_rr.352000000a927e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_337_rr.352000000a927e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808899
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA131800.092.573711
TAGTAAC138200.087.0013662
CGCACTT39650.079.5589145
GTAACAA153850.078.46944
TAACAAG180450.066.429165
AGTAACA185500.065.4274143
ACGCACT55150.057.8429154
AGCACGC52350.057.8273621
GCACGCA53250.056.5819052
AAGAGCG38100.053.3337829
CTCGCTC32300.044.993653
TGACGCC18400.044.09961145
ACAAGAG277150.043.5336657
CAAGAGC265600.043.5062338
CACGCAC72200.042.5158233
GTCTCGC35200.042.0945471
AACAAGA286900.041.9069446
AAACACG76600.041.7368665
TCGCTCT36700.040.1772734
AACACGA80900.039.1724931
Produced by FastQC (version 0.11.2)