FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_328_rr.352000000a9261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_328_rr.352000000a9261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774962
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT263440.3888435093805692No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC114560.1690931993419299No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC102380.15111523872753826No Hit
GGATCCTTTTACTAGTTTTCCTTCTTGGGTTCCAACACTGAAAGTTTTGG96740.14279046878786922No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG95990.1416834515086579No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG95770.14135872644008926No Hit
GTGCATCAAACATTTAGTAAATATTACTCGTAGTGCTTTTCCAAAGGATC89210.1316760153045877No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT82240.12138813472311727No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC81980.12100436873299068No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC80680.11908553878235775No Hit
GGGGCTATTTACTCTGTCTCCCTTGAAACAAGCTTTGGTCATGTCATATC74270.10962423110269845No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT71450.10546184613286391No Hit
GTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT70040.10338065364794664No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT69990.10330685249599925No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA69710.1028935660450937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG26050.069.663185
AACACGA27000.060.8384636
ACACGAG28300.057.7922947
ACGAGCC11000.055.9953169
CACGAGC29750.055.21278
GCCGACC2950.054.42016145
TGACGCC4000.051.939243145
GAAACAC36050.050.538024
CGACGAA8400.048.341717145
AGAAGCG19300.045.177155
CGGTGCA11300.044.5456772
TTATTCA92850.043.920349
ACGAGCG856.148371E-541.646289
ATCCTGC37200.040.6172145
TATCGGG1653.0214142E-740.063328145
GCACGCA4350.039.115392
GTGTCCT109700.037.6790961
GTAACAA107250.037.3457764
CGCACTT4250.036.694825
AAGCGGT16300.036.4868287
Produced by FastQC (version 0.11.2)