FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_325_rr.352000000a9255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_325_rr.352000000a9255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7222447
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT25600.065.347195
ACGCACT34950.051.9233474
AGCACGC35900.050.752431
TAGTAAC67100.049.8687632
ACGAGCG3600.047.1967779
CCATACG19450.045.191525
GTAGTAA77650.044.737611
CACGCAC42100.043.9496573
CACGAGC32300.043.399778
GCACGCA43850.041.8735052
CGACGAA4800.039.344013145
ACACGAG37600.037.0957537
AACACGA38200.037.069396
TAACAAG96700.034.159725
TACGTGC27350.033.910858
AAACACG48950.032.5825235
GTAACAA102250.032.3066944
AGTAACA115300.029.3897323
ATTAGTA66150.026.6862561
ACGAGCT14800.025.3523259
Produced by FastQC (version 0.11.2)